eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2026-05-11 14 1 134 145 26929 article Saylee Vilas Kalekar 1 Niranjan Prakashrao Patil 2 Rajashree Bhalchandra Patwardhan 3 Department of Microbiology, Fergusson College (Autonomous), Affiliated to Savitribai Phule Pune University, Shivajinagar, Pune, Maharashtra, India Department of Microbiology, MES Abasaheb Garware College, Affiliated to Savitribai Phule Pune University, Pune, Maharashtra, India Department of Microbiology, HaribhaiV.Desai College of Arts, Science and Commerce, Affiliated to SavitribaiPhule Pune University,Budhwarpeth, Maharashtra, India. Sustainable agricultural practices is a need of time to support the supply of agricultural produce for continuously growing global population and minimizing the dependence on chemicals. Plant growth promoting rhizobacteria (PGPR) invade the rhizosphere and use a variety of direct and indirect methods to promote plant growth. In this study,thirty-one PGPR were isolated and screened.Four isolates that had greatest number of characteristics that promote plant growth were selected. The 16S rRNA gene sequencing method was used to identify the selected isolates and they were identified as Proteus vulgaris PT2, Pseudomonas putida NP12, Serratia rubidae NW19 and Pseudomonas fulva TD4. Proteus vulgaris PT2 showed maximum IAA production of 127.65 µg/mL followed by Pseudomonas fulva TD4 showing 98 µg/mL.The impact of selected isolates on Vigna radiataplantseeds was examinedby calculatingvigour index using parameters like seed germination percentage, root length and shoot length. Pseudomonas fulva TD4 exhibited a notable increase in germination percentage 96.66 % and Vigor index of 2273.153in comparison to control group. The isolation and characterization of potent rhizobacterial strains highlight their role as eco-friendly biofertilizers, promoting environmentally safe crop production. Also, the high IAA production by Proteus vulgaris PT2 and Pseudomonas fulva TD4 demonstrates their potential in enhancing plant growth and root development, which is critical for nutrient uptake. Overall, Pseudomonas fulva TD4emerged as a promising biofertilizer candidate with potential application in sustainable crop production system. The significant improvement in germination percentage and vigour index of Vigna radiata indicates the practical applicability of these isolates in seed treatment and crop yield enhancement. http://www.agriculturejournal.org/volume14number1/isolation-and-identification-of-multifarious-pgpr-from-rhizosphere-and-its-effect-on-plant-growth-promotion-of-vigna-radiata/ Biofertilizer; IAA; PGPR; Pseudomonas fulva; Vigna radiata