eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2018-04-20 6 1 08 19 3606 article Muhammed Ali Hossain 1 Ahsan Habib 2 Mohammad Shafiqul Islam 3 Fatema -Tuz - Zohura 4 Md. Atiqur Rahman Khokon 5 Department of Plant Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. Department of Agriculture, Noakhali Science and Technology University, Noakhali, Bangladesh. Alternaria leaf spot or black leaf spot caused by Alternaria brassicicola is the devastating diseases of mustard-rapeseed in Bangladesh, and can cause yield loss up to 60% every year. A total of 27 cultivars of Brassica spp. where Brassica rapa (13), B.  juncea (5) and B. napus (9) were selected for resistance screening against A. brassicicola. Two inoculation methods viz. detached leaf and seedling inoculation were assessed for checking the validation of inoculation technique using cultivated mustard-rapeseed varieties in Bangladesh a method to measure resistance to A. brassicicola.  A significant positive correlation between the results of two inoculation methods was found in this study. The detached leaf  technique was more suitable  due to development of  clear symptoms on the leaves within 36 hrs, and suitable for  screening large scale genotypes  for resistance. The 3rd leaves of 30 day-old were more suitable for inoculation having severe symptoms than the 4th leaves. Among 27 Bangladeshi mustard-rapeseed cultivars all cultivars expressed susceptible reaction to A. brassicicola, except BINA Sharisha-8, as it had border line resistance. http://www.agriculturejournal.org/volume6number1/validation-of-inoculation-test-and-screening-for-resistance-sources-of-mustard-rapeseed-cultivars-in-bangladesh-against-alternaria-brassicicola/ Resistance Screening; Alternaria Brassicicola; Mustard-Rapeseed; Inoculation Test; Validation of Test