eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2019-04-25 7 1 37 45 6438 article Spyridon Mantzoukas 1,2 Nikoleta-Kleio Denaxa 1,3 Ioannis Lagogiannis 2 Department of Agricultural Technology, Technological Institute of Western Greece, Amaliada. Department of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece. Faculty of Crop Science, Laboratory of Pomology, Agricultural University of Athens, Iera Odos, Athens, Greece. Susceptibility of Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) populations to Metarhizium anisopliae and Bacillus thuringiensis (Bt) were evaluated under laboratory conditions. T. absoluta larvae were treated either individually or in combination with a single dose rate of B. thuringiensis (0.5 μL/L) and three conidial suspensions viz. 1x10⁴, 1x10⁶ and 1x10⁸ spores/mL of M. anisopliae. Larval mortality, pupation, adult emergence, mycosis and sporulation varied depending on the application of different quantities of M. anisopliae alone and in combination with B. thuringiensis. Maximum mortality (100%) was achieved in 2^nd instar larvae when M. anisopliae (1x10⁸ spores/mL) and B. thuringiensis (0.5µL/L) were applied synergistically,while 4^th instar larvae recorded a 95.45% mortality. Compared with the untreated checks, mortality, pupation and adult emergence of both 2^nd and 4^th instar larvae were significantly reduced with the combined application of M. anisopliae (1x10⁸ spores/mL) and B. thuringiensis (0.5µL/L). Μycosis was most prevalent on 2^nd and 4^th instar larvae, exceeding 88 and 80% respectively,after exposure to 1×10⁴ conidia/mL. The results indicate that the entomopathogenic fungi and the insecticidal protein produced by B. thuringiensis can be used in combination as biocontrol agents for the management of T. absoluta. http://www.agriculturejournal.org/volume7number1/efficacy-of-metarhiziumanisopliae-and-bacillus-thuringiensis-against-tomato-leafminer-tuta-absoluta-meyrick-lepidoptera-gelechiidae/ Bacillus Thuringiensis; Biological Control; Metarhizium Anisopliae; Tomato; Tuta Absoluta