eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2019-04-25 7 1 90 98 6509 article Pooja Patil 1 Swarupa Agnihotri 1 Department of Botany, Viva collegeVirar (Mumbai), India In the fields, it is commonly observed that fenugreek plants are not growing to their maturity in the vicinity of TrianthemaportlacastrumL. In this context, the present study was conducted in the laboratory to observe allelopathic interaction between Trianthemaand fenugreek. Aqueous extracts of seed, stem and leaf of Trianthemawere made. Treatment of these aqueous extracts was given to fenugreek seeds. Germination study was performed by using Petri plates. All the experiments were performed in triplicates. Pre-treatment of aqueous extracts of seed, stem and leaf of Trianthema. had caused a decline in germination percentage, fresh weight, radical and coleoptile length of fenugreek seeds when compared to untreated seeds. At the same time, mean germination time was found to be more in pre-treated seeds compared to control seeds. After 24 hrs of treatment, the enzymatic study was performed. Enzymes from antioxidant metabolism such as peroxidase, ascorbate peroxidase, superoxide dismutase and activity of DPPH was reduced in treated seeds than control seeds. The activity of catalase was increased after treatment of leachates. All the data is graphically presented with the standard deviation. Exogenous treatment of aqueous extracts of Trianthemahas caused variation in saponin content of fenugreek seeds. This observation was recorded by using finger prints of HPTLC. Finger prints showed that content of saponin was also declined in treated seeds as compared to control. All these observations indicate the strong negative influence of Trianthemaon growth and metabolism of fenugreek. http://www.agriculturejournal.org/volume7number1/study-of-seed-germination-and-enzyme-metabolism-during-allelopathic-interaction-of-trianthemaportulacastrum-l-and-fenugreek-seeds/ Allelopathy; Fenugreek; Trianthemaportulacastrum L.