eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2019-08-25 7 2 181 188 7252 article Sangeeta Sankhalkar 1 Richa Komarpant 1 Tanvi Raut Dessai 1 Jennis Simoes 1 Sunita Sharma 1 Department of Botany, Parvatibai Chowgule College of Arts and Science (Autonomous), Margao Goa, India. Agriculture has been on the forefront of every country’s economy. In the recent years hydroponic has emerged as a new technology to grow plants. It requires less space, adds greenery around, easy to be practiced indoors and on open spaces. Although hydroponic is now being practiced with total success, not many studies are done on the physiology and biochemistry of the plant grown. The present work was planned with an objective to undertake physiological and biochemical studies such as relative water content (RWC), root shoot ratio, chlorophyll content, sugars and protein in vegetable plants (pea, okra and moong) grown with soil and soil-less cultures (hydroponics). Our results showed higher root ratio in plants grown in hydroponics than in the soil. A slight variation in relative water content (RWC) was observed. Chlorophyll content was found to be higher in okra and moong plants grown in soilless culture. Total Sugar and protein content in soil grown okara & moong seedlings were slightly higher in comparison to those grown hydroponically indicating that soil is best medium for plant growth. Physiology of hydroponically grown plants requires more research work as it is an alternative technology to grow plants in lands where soils are non fertile or where space or agriculture is a constrain. http://www.agriculturejournal.org/volume7number2/effects-of-soil-and-soil-less-culture-on-morphology-physiology-and-biochemical-studies-of-vegetable-plants/ Chlorophyll Content; Hydroponics; Protein Analysis; Root-Shoot Ratio; Soil Culture