eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2021-12-30 9 3 179 187 10831 article Melusi Rampart * Demel Teketay Kamogelo Makgobota Witness Mojeremane Ronnie Mmolotsi Topoyame Makoi Department of Range and Forest Resources, Faculty of Natural Resources Botswana University of Agriculture and Natural Resources, Private Bag 0027, Gaborone, Botswana. Cassia abbreviata and Senegalia nigrescens are indigenous trees found in North and Central regions of Botswana. However, inadequate knowledge of their silviculture and decline in population due to deforestation, expanding settlements, infrastructure and agriculture are major threats to most indigenous tree species in Botswana. Like many indigenous tree species, Cassia abbreviata and Senegalia nigrescens are slow-growing and are threatened by overexploitation for numerous uses. Because of the increased demand for medicinal uses, medicinal species such Cassia abbreviata are rapidly disappearing in many habitats and threatened to extinction.  This study assessed the response of pre-sowing treatment methods on quiescency and germination of seeds of Cassia abbreviata and Senegalia nigrescens tree species found in semiarid Savanna Ecozone of Botswana. Pre-sowing treatment included immersion in 98% undiluted sulfuric acid (H₂SO₄) for 15, 30, 45 and 60 minutes, submergence in boiling water for one, two, three minutes and submerged in warm water for 24 hours, mechanical scarification of the seed coat and control. ANOVA showed that there is a highly significant difference (P < 0.0001) among the treatments of C. abbreviata in seed germination and no significant difference among the treatments of S. nigrescens. The highest germination percentage of C. abbreviata seeds was 81%, which was found from seeds immersed in H₂SO₄ for 30 minutes, while for S. nigrescens seeds, the highest germination percentage was 99%, which was found from seeds treated with mechanical scarification and those immersed in H₂SO₄ for 15 and 45 minutes. The objective of this study is to determine the effect of pretreatment methods on germination of C. abbreviata and S. nigrescens seeds. Based on these results, we recommend sulphuric acid and mechanical scarification as suitable seed pre-sowing treatments for enhancing the germination of C. abbreviata and S. nigrescens, respectively. http://www.agriculturejournal.org/volume9number3/enhancing-germination-of-seeds-of-cassia-abbreviata-and-senegalia-nigrescens-using-pre-sowing-seed-treatments-in-botswana/ Germination Percentage; Germination Rate; Seed Characteristics; Seed Dormancy