eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2021-12-30 9 3 171 178 10845 article Happiness Nyambuge Msenya 1* Steven Runo 2 Douglas Watuku Miano 3 Mary Wanjiku Gikungu 4 Elijah Kathurima Gichuru 1 Cecilia Wagikondi Kathurima 1 Kenya Agricultural and Livestock Research Organization-Coffee Research Institute, Ruiru-Kenya. Kenyatta University, Department of Biochemistry and Biotechnology, Nairobi, Kenya. The University of Nairobi, Department of Plant Science and Crop Protection, Kangemi, Kenya. National Museums of Kenya, Department of Zoology, Nairobi, Kenya. Colletotrichum kahawae is a causative fungal agent of Coffee Berry Disease (CBD) that negatively affects coffee production. Its control relies on use of resistant varieties and chemical control. Fungicides used in control of CBD are costly and pose a risk to environment and health. The study was aimed at assessing the possible antagonistic potential of fungal isolates against Colletotrichum kahawae in in vitro conditions. Five isolates were used; one isolate was collected from berries while the rest were collected from soil. Testing for antagonism against Colletorichum kahawae was done by co-culturing the isolates on Potato Dextrose Agar. The degree of antagonism was determined by measuring and comparing the radial growth of pathogen with the bio-agent against the control. Out of the five isolates tested against C. kahawae, three isolates Fusarium proliferetum, Penicillium and Fusarium ceraneasum significantly inhibited test pathogen growth at 60%, 55% and 45.45%, respectively. The other isolates, Fumigatus aspergillus and Chaetomium perithecia showed inhibition of growth at 40% and 18.18% respectively. It was concluded that there is potential of using fungi as bio control of the coffee berry disease. http://www.agriculturejournal.org/volume9number3/the-potential-of-fungi-as-bio-control-agents-in-managing-colletotrichum-kahawae-in-kenya/ Antagonist; Bio-Control; Coffee Berry Disease; Colletotrichum Kahawae