eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2023-01-05 10 3 277 289 12298 article Sneka, M. 1 Shanmitha, A. R. 1 Siva Priyadharshini, A 1 Thilakavathi, G 1 Joselin, J. 1 Sarenya, R 1 Department of Agricultural Microbiology, Sethu Bhaskara Agricultural College and Research Foundation (Affiliated to Tamil Nadu Agricultural University), Karaikudi, Sivaganga-India Department of Agricultural Microbiology, Pandit Jawaharlal Nehru College of Agricultural And Research Institute, Karaikal, Union Territory of Puducherry, India Nitrogen is a crucial component that acts as building blocks for nucleic acids and proteins. It is abundantly present in atmosphere of the earth but  plants are not able to easily utilize it. Diazotrophic microorganisms such as Rhizobium convert N₂ and make it available to pulse crops. In the present study, a total of thirty-four Rhizobium isolates were recovered, from that twenty-two with gram-negative-rods were biochemically characterized. Most of the isolates were negative for congo red (CR) absorption, glucose-peptone, lactose, and hofer’s alkaline assays. These isolates have PGP (plant growth promoting) properties such as solubilisation of mineral, growth hormone, and hydrogen cyanide (HCN) productions along with antagonistic activity. Plant study revealed that SBGR25 treatment has highest germination per cent (98.0 %), shoot (10.3 cm) and total-height (16.6 cm), and dry weight (5.60 g). Hence, the present study suggests that SBGR25 strains could be used as promising bioinoculants for the pulse crop. https://www.agriculturejournal.org/volume10number3/isolation-and-characterization-of-rhizobium-from-green-gram-vigna-radiata/ Biological Nitrogen Fixation; Plant Growth Promotion; Rhizobium; Sustainable Agriculture