eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2024-08-30 12 2 705 713 20101 article Anamika Singh 1 Rakesh Kumar Verma 1 Department of Biosciences, School of Liberal Arts and Sciences, Mody University of Science and Technology, Lakshmangarh, Sikar (Rajasthan), India. Agriculture has recently prioritized Plant Growth Promoting Rhizobacteria (PGPR) due to their significance in the rhizosphere, which is an ecological unit in the biosphere. Isolating PGPR from Cicer arietinum (chickpea) nodules and characterizing its PGP activities was the purpose of the present study. The research showed that the specific strain could manufacture several useful chemicals, such as hydrogen cyanide (HCN), ammonia, siderophore, indole acetic acid (IAA) and nitrogenase. Furthermore, the isolate was identified as Bacillus licheniformis AS11 through 16s rRNA analysis, and alignment analysis showed 99% similarity with the Bacillus licheniformis KPA12 isolate. It's worth highlighting that, Bacillus licheniformis AS11 a potential PGPR, is considered a valuable asset for agriculture when it has a positive effect on plant growth. Therefore, Bacillus licheniformis AS11 can be a beneficial and constructive addition to the field of agriculture. These findings suggest potential applications in sustainable agriculture by improving crop yield through natural growth-promoting mechanisms and reducing reliance on chemical fertilizers. https://www.agriculturejournal.org/volume12number2/molecular-characterization-of-plant-growth-promoting-rhizobacteria-isolated-from-nodules-of-cicer-arietinum-plant/ IAA Production; PGPR; Phylogenetic analysis; Rhizosphere;16srRNA identification