eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2024-08-30 12 2 750 761 20547 article Utkarsh Singh Rathore 1 Rudra Pratap Singh 1 Sonika Pandey 2 R.K. Mishra 2 Division of Plant Pathology, Bhagwant University, Ajmer, Rajasthan, India Division of Crop Protection, ICAR-Indian Institute of Pulses Research, Kanpur, India The aim of this study was to find and characterize Trichoderma isolates by antagonistic and enzymatic activity to evaluate their potential as biocontrol agents against Dry root rot (DRR). Trichoderma isolates were isolated from thepulses rhizosphere of different districts of Uttar Pradesh. Twenty one Trichoderma isolates were identified using ribosomal DNA internal transcribed spacer (ITS) regions and translation elongation factor 1-alpha (tef1). In addition, enzymatic profiling of Trichoderma isolates was done indicated strong cell wall degrading enzyme activities and plant growth promoting traits of Trichoderma isolates. Overall, our results suggested that the isolated Trichoderma spp. have prodigious potential for plant growth promotion and can be used as biocontrol agents against dry root rot. https://www.agriculturejournal.org/volume12number2/molecular-identification-antagonistic-assay-and-enzyme-profiling-of-selected-trichoderma-isolates/ Biocontrol; Chickpea; Growth promotion; Pulses; Trichoderma