eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2025-04-30 13 1 198 212 24627 article Zeiwang Konyak 1 Madhu Kamle 1 Pradeep Kumar 2 Applied Microbiology Laboratory, Department of Forestry, North Eastern Regional Institute of Science and Technology, Nirjuli, India Department of Biochemistry, University of Lucknow, Lucnow, Uttar Pradesh, India. The prolonged use of artificial fertilizers and agrochemicals damages soil health by reducing water retention, increasing salinity, and disrupting nutrient balance. This highlights the need for eco-friendly alternatives to manage crop diseases. Plant growth promoting rhizobacteria (PGPR) are beneficial soil bacteria that enhance plant growth, suppress pathogens, and restore soil fertility. They produce key compounds like phytohormones, antimicrobial metabolites, and enzymes, making them promising biopesticides and biofertilizers. Additionally, termite mound soil, rich in nutrients and beneficial microorganisms, supports plant growth and crop yield. Bacillus spp. is considered the most suitable PGPR with multiple plant growth promoting (PGP) activities. In this study, 42 soil-inhabiting termitarium bacteria were isolated and screened for their antifungal activity against A. alternata and their plant-growth-promoting activity in vitro. TH5/J showed maximum percentage inhibition of the phyto-pathogen (35.06 %) after 48 hours. CFCF (cell-free culture filtrate) also inhibited A. alternata in vitro. TH5/J produced the highest amount of Indole Acetic Acid (IAA), which supports the PGP in plants. The isolates also tested positively for ammonia and hydrogen cyanide (HCN). TH2/2 and TH5/J showed the maximum PGP activity on the mung bean seeds in vitro. These isolates were identified as belonging to Bacillus spp through biochemical analysis. This study highlights Bacillus spp. from termite mounds as eco-friendly biopesticides and biofertilizers, promoting plant growth of mung beans (Vigna radiata L.) and suppressing A. alternata. It emphasizes termite mound soil as a reservoir of beneficial microbes, offering a sustainable alternative to synthetic agrochemicals. https://www.agriculturejournal.org/volume13number1/harnessing-termitarium-associated-bacillus-species-for-biocontrol-and-plant-growth-promotion/ Ammonia (NH3); Cell-free culture filtrate (CFCF); Hydrogen cyanide (HCN); Indole acetic acid (IAA); Plant growth promoting (PGP); Termiterium Hill (TH); Volatile organic compounds (VOCs)