eng Enviro Research Publishers Current Agriculture Research Journal 2347-4688 2321-9971 2026-01-10 13 3 857 864 25975 article Manisha Thakur 1 Guruprasad Kadur Narayan 1 Uttam Sharma 2 Kundan Viliya 2 Department of Life Science, Shri Vaishnav Institute of Science, SVVV, Indore, India. Department of Physics, Shri Vaishnav Institute of Science, SVVV, Indore, India. The impact of pre-treating maize crop (Zea mays: GSF.2) seeds with Gaseous plasma utilizing different gases has been investigated. A gaseous glow discharge non-thermal plasma was produced in a plasma system, utilizing an RF power supply, with N₂, He, Ar, and H₂ as feeding gases. A dose-responsive curve with different exposure times and power input showed an optimum enhancement of seedling growth after exposure for 240 seconds at 30 W power input. Maximum enhancement in growth was obtained with N₂ plasma (55% in seedling length). Promotion in growth was lower with other gases comparatively (He, H₂, Ar). Biochemical analysis showed a rise in the level of H₂O₂ in the seeds exposed to plasma. This was followed by an elevated activity of the enzyme α-amylase, which degrades starch, the reserve food material in maize seeds. H₂O₂ acts as a signalling molecule to enhance the activity of the α-amylase enzyme. It is concluded that exposure of seeds to plasma alters pre-germination metabolism in maize seeds that results in enhanced growth of the seedlings. https://www.agriculturejournal.org/volume13number3/influence-of-plasma-on-pre-germination-metabolism-of-maize/ α-amylase; Germination; H₂O_2; Maize; Plasma