Isolation and Identification of Multifarious PGPR from Rhizosphere and its Effect on Plant Growth Promotion of Vigna Radiata.

Saylee Vilas Kalekar¹, Niranjan Prakashrao Patil² and Rajashree Bhalchandra Patwardhan^3*

¹Department of Microbiology, Fergusson College (Autonomous), Affiliated to Savitribai Phule Pune University, Shivajinagar, Pune, Maharashtra, India

²Department of Microbiology, MES Abasaheb Garware College, Affiliated to SavitribaiPhule Pune University, Pune, Maharashtra, India

³Department of Microbiology, HaribhaiV.Desai College of Arts, Science and Commerce, Affiliated to SavitribaiPhule Pune University,Budhwarpeth, Maharashtra, India.

Corresponding authorE-mail:dr.rbpatwardhan@gmail.com

DOI : http://dx.doi.org/10.12944/CARJ.14.1.10

Article Publishing History

Received: 23 Mar 2026
Accepted: 15 Apr 2026
Published Online: 23 Apr 2026

Review Details

Reviewed by: Dr. Aparna Gunjal
Second Review by: Dr. Mohammed Al Jawasim
Final Approval by: Dr. José Luis da Silva Nunes

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Abstract:

Sustainable agricultural practices is a need of time to support the supply of agricultural produce for continuously growing global population and minimizing the dependence on chemicals. Plant growth promoting rhizobacteria (PGPR) invade the rhizosphere and use a variety of direct and indirect methods to promote plant growth. In this study,thirty-one PGPR were isolated and screened.Four isolates that had greatest number of characteristics that promote plant growth were selected. The 16S rRNA gene sequencing method was used to identify the selected isolates and they were identified as Proteus vulgaris PT2, Pseudomonas putida NP12, Serratia rubidae NW19 and Pseudomonas fulva TD4. Proteus vulgaris PT2 showed maximum IAA production of 127.65 µg/mL followed by Pseudomonas fulva TD4 showing 98 µg/mL.The impact of selected isolates on Vigna radiataplantseeds was examinedby calculatingvigour index using parameters like seed germination percentage, root length and shoot length. Pseudomonas fulva TD4 exhibited a notable increase in germination percentage 96.66 % and Vigor index of 2273.153in comparison to control group. The isolation and characterization of potent rhizobacterial strains highlight their role as eco-friendly biofertilizers, promoting environmentally safe crop production. Also, the high IAA production by Proteus vulgaris PT2 and Pseudomonas fulva TD4 demonstrates their potential in enhancing plant growth and root development, which is critical for nutrient uptake. Overall, Pseudomonas fulva TD4emerged as a promising biofertilizer candidate with potential application in sustainable crop production system. The significant improvement in germination percentage and vigour index of Vigna radiata indicates the practical applicability of these isolates in seed treatment and crop yield enhancement.

Keywords:

Biofertilizer; IAA; PGPR; Pseudomonas fulva; Vigna radiata

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