Optimization of Pectinase Production by Aspergillus Oryzae RR 103

Ravi.Ketipally, M.Raghu Ram^*

Department of Botany and Microbiology, Acharya Nagarjuna University Nagarjuna Nagar, Guntur, India.

Corresponding author Email: mraghuram2002@gmail.com

DOI : http://dx.doi.org/10.12944/CARJ.6.1.05

Abstract:

The purpose of this investigation is to isolate and Exploit diversified microorganisms from mangrove habitat and their commercial utilization in industrial sector. Pectinases are enzymes having a broad Industrial and commercial applications. Polygalacturonase produced by microorganisms which breakdown pectin, present in plant material to simple chemical substances. The enzymatic breakdown of α-1, 4-glycosidic linkages in polygalacturonic acid is catalysed by Polygalacturonic acid, releasing D-galacturonate which is a simple chemical substance. The production of Polygalacturonase by Aspergillus oryzae RR103 was enhanced by optimization of different nutritional parameters. Highest polygalacturonase activity was observed at 192h at 35°C. Optimum pH for the production of enhanced Polygalacturonic acid was at pH 6.0. The carbon and nitrogen sources which gave enhanced enzyme activity were Cellulose and Malt extract respectively. Highest enzyme specificity was recorded when the substrate used was citrus pectin. Polygalacturonase from Aspergillus oryzae RR 103 is suitable for industrial scale production as it has high productivity.

Keywords:

Aspergillus oryzae RR 103; Mangrove; Optimization; Polygalacturonase

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